Journal: Frontiers in Immunology

Article Title: Stem cell factor 248 shapes ILC2 transcriptional programs and promotes mucosal inflammation in allergic asthma

doi: 10.3389/fimmu.2026.1843080

Figure Lengend Snippet: SCF248 expression impacts ILCp-ILC2 lineage specification in vitro . Bone marrow ILCp were sorted from naive Balb/c male mice. (A) ILCp sorting strategy. (B) SCF248 proinflammatory isoform was upregulated in OP9DL1 cells treated with rmIL-13 (10ng/mL for 16h). Gene expression analysis by qPCR was performed on ILCp cells differentiated in vitro for 72 h on OP9-DL1 cells or OP9-DL1 cells treated with IL-13. Cells were washed prior to co-culture. (C-I) Inhibitor of DNA binding 2 ( ID2 ), Gata3 , CD90 , Icos , c-Kit , Il4 , Il13 . (J) flow cytometry analysis of differentiated ILC2. Data are presented as mean ± SEM. Experiments were performed twice with n = 3–4 replicates per group. Statistical significance was determined using ordinary two-way ANOVA. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.001.

Article Snippet: Next, we enriched our progenitor population by magnetically isolating Lineage + cells using a Direct Lineage Cell Depletion Kit and following the manufacturer’s instructions (Miltenyi Biotec).

Techniques: Expressing, In Vitro, Gene Expression, Co-Culture Assay, Binding Assay, Flow Cytometry